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Lnc RNA ‐regulated Infection and Inflammation Pathways Associated with Pregnancy Loss: Genome Wide Differential Expression of lnc RNA s in Early Spontaneous Abortion
Author(s) -
Wang Hong,
Cao Qingying,
Ge Jun,
Liu Chunmiao,
Ma Yanhong,
Meng Yuciu,
Wang Yuxin,
Zhao Xiaoli,
Liu Ru,
Li Caixia,
Wang Yu,
Zhong Julia,
Ju Weina,
Jenkins Edmund C.,
Brown W. Ted,
Zhong Nanbert
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12275
Subject(s) - rna , biology , pregnancy , inflammation , antisense rna , abortion , decidua , andrology , microbiology and biotechnology , bioinformatics , genetics , gene , immunology , medicine , fetus , placenta
Problem Spontaneous abortion ( SA ) occurs before 20 gestational weeks. Approximately, half of recurrent SA has no identifiable cause. No report has yet been investigated the possible involvement of lnc RNA in pregnancy loss. Method of Study Sixteen pairs of pregnancies with spontaneous abortions ( SA ) and induced abortions ( IA ) were studied. Embryonic sacs and decidua were collected for each pregnancy. A Human Lnc RNA Array was employed to profile genomewide lnc RNA s, which were then validated by RT ‐ PCR . Results Differentially expressed lnc RNA s were identified. Biological pathways were categorized into six major groups: infection and inflammation, metabolism, signaling and transcriptional regulation, smooth muscle contraction, cell process, and coagulation. Conclusions Infection and inflammation pathways regulated by lnc RNA s were determined as the predominant pathogenetic factors underlying the SA . Finding that antisense lnc RNA s have been either up‐ or down‐regulated suggests that they may have both cis‐ and trans ‐regulations.