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Conjugated Linoleic Acids Attenuate LPS ‐Induced Pro‐Inflammatory Gene Expression by Inhibiting the NF ‐κB Translocation Through PPAR γ in Buffalo Granulosa Cells
Author(s) -
Yenuganti Vengala Rao,
Ravinder Ravinder,
Singh Dheer
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12261
Subject(s) - lipopolysaccharide , gene expression , nf κb , inflammation , signal transduction , immune system , granulosa cell , chemistry , endocrinology , biology , medicine , microbiology and biotechnology , gene , immunology , ovary , biochemistry
Problem In granulosa cells, TLR 4‐mediated LPS ‐induced immune response interferes with ovarian granulosa cell function. Method of study LPS ‐induced pro‐inflammatory gene expression was monitored by real‐time PCR and NF ‐κB was determined by the immuno‐blotting and immuno‐staining in granulosa cells in vitro . Results The LPS (1 μg/mL) increased pro‐inflammatory gene expression which was reverted back by treatment with TLR 4 signaling inhibitor. Cotreatment of CLA (10 μ m ) with LPS did not show any effect on LPS ‐induced pro‐inflammatory gene expression but granulosa cells pre‐treated with CLA for 24 hr, attenuated LPS ‐induced pro‐inflammatory gene expression and nuclear NF ‐κB. GW 9662, a PPAR γ‐inhibitor, further increased the expression of pro‐inflammatory genes. Conclusion The present findings reiterated that pre‐treatment with CLA can prevent LPS ‐induced granulosa cells dysfunction.