Premium
Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR ‐1
Author(s) -
Fu Qiang,
Tao Yu,
Piao Hailan,
Du MeiRong,
Li DaJin
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12211
Subject(s) - stromal cell , microbiology and biotechnology , decidual cells , chemistry , decidua , cell , biology , cancer research , placenta , pregnancy , biochemistry , genetics , fetus
Problem To determine the effect of collagen from maternal–fetal interface on decidual natural killer cell (d NK ) function. Method of study Decidual and villous samples were collected from normal pregnancy and miscarriage. The phenotype and cytokine production were analyzed, respectively, by flow cytometry and enzyme‐linked immunosorbent assay ( ELISA ). Co‐culture was established to investigate the effect of trophoblasts and decidual stromal cells ( DSC s) on d NK s. Results Maternal–fetal interface of normal pregnancy showed higher collagen and LAIR ‐1 expression than that of miscarriage. Co‐culture of d NK s with HTR ‐8/ DSC s up‐regulated LAIR ‐1 on d NK s that could be attenuated by pre‐treatment with LAIR ‐2, a competitive inhibitor of LAIR ‐1. Collagen down‐regulated expression of cell surface receptor activity and intracellular perforin, while it up‐regulated expression of suppressive receptor on d NK s. Co‐culture of d NK s with HTR ‐8/ DSC s decreased perforin expression and T h1‐type cytokines production by d NK s, which could be abrogated by LAIR ‐2. In addition, silence of collagen in HTR ‐8/ DSC s by sh RNA significantly attenuated regulation on d NK s. Conclusion Trophoblasts and DSC s regulate decidual NK cell functions via secreting collagen, which is involved in the maintenance of human pregnancy.