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Expression of Natural Cytotoxicity Receptors on Peritoneal Fluid Natural Killer Cell and Cytokine Production by Peritoneal Fluid Natural Killer Cell in Women with Endometriosis
Author(s) -
Funamizu Ayano,
Fukui Atsushi,
Kamoi Mai,
Fuchinoue Kohei,
Yokota Megumi,
Fukuhara Rie,
Mizunuma Hideki
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12206
Subject(s) - peritoneal fluid , endometriosis , cytokine , natural killer cell , tumor necrosis factor alpha , lymphokine activated killer cell , janus kinase 3 , interleukin 21 , flow cytometry , immunology , medicine , biology , cytotoxicity , cancer research , immune system , t cell , in vitro , biochemistry
Problem To investigate the relationship between the expression of natural cytotoxicity receptors ( NCR s) on peritoneal fluid ( PF ) natural killer ( NK ) (pf NK ) cells and cytokine production by pf NK cells in women with endometriosis. Method of study Peritoneal fluid was collected from women with endometriosis undergoing laparoscopic surgery ( n = 21) and controls without endometriosis ( n = 28). The expression of NK cell surface antigens such as CD 16 and NCR s ( NK p46, NK p44 and NK p30) on pf NK cells, and cytokines production by pf NK cells [tumor necrosis factor ( TNF )‐α, IFN ‐γ, IL ‐4, IL ‐10, GM ‐ CSF and transforming growth factor ( TGF )‐β 1 ] were measured using multicolor flow cytometry. Results The percentages of CD 56 + / NK p46 + cells and CD 56 dim / NK p46 + cells in severe endometriosis group were significantly lower than that in controls. TNF ‐α and IFN ‐γ production by pf NK cells in severe endometriosis group was significantly higher than those in controls. Conclusion The differential expression of NK p46, TNF ‐α, and IFN ‐γ on pf NK cells in women with severe endometriosis may allow the proliferation and angiogenesis of endometriotic cells.