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Effect of Hydroxychloroquine on Antiphospholipid Antibody‐Induced Changes in First Trimester Trophoblast Function
Author(s) -
Albert Caroline R.,
Schlesinger William J.,
Viall Chez A.,
Mulla Melissa J.,
Brosens Jan J.,
Chamley Lawrence W.,
Abrahams Vikki M.
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12184
Subject(s) - hydroxychloroquine , trophoblast , secretion , antibody , antiphospholipid syndrome , medicine , endocrinology , immunology , pregnancy , andrology , biology , placenta , fetus , disease , covid-19 , infectious disease (medical specialty) , genetics
Problem Women with antiphospholipid syndrome ( APS ) are at risk for pregnancy complications. Antiphospholipid antibodies (a PL ) alter trophoblast function by triggering an inflammatory cytokine response; modulating angiogenic factor secretion; and inhibiting migration. While patients with APS are often treated with hydroxychloroquine ( HCQ ), its effect on trophoblast function is poorly understood. Method of study A human first trimester trophoblast cell line was treated with or without antihuman β 2 GPI m A bs in the presence or absence of HCQ . Supernatants were analyzed by ELISA . Cell migration was measured using a colormetric assay. Results Antiphospholipid antibodies‐induced trophoblast IL‐8, IL‐1 β, PlGF, and s E ndoglin secretion were not altered by HCQ . a PL ‐induced inhibition of trophoblast migration was partially reversed by HCQ, even though HCQ significantly increased secretion of pro‐migratory IL‐6 to greater than baseline. a PL ‐induced upregulation of TIMP2 appears to inhibit trophoblast migration; the inability of HCQ to prevent a PL ‐induced TIMP2 may explain why migration was only partially restored. Conclusion Hydroxychloroquine reversed the a PL ‐inhibition of trophoblast IL‐6 secretion and partially limited a PL ‐inhibition of cell migration. Thus, some form of combination therapy that includes HCQ may be beneficial to pregnant APS patients.