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Choriodecidual Cells From Term Human Pregnancies Show Distinctive Functional Properties Related to the Induction of Labor
Author(s) -
CastilloCastrejon Marisol,
MerazCruz Noemí,
GomezLopez Nardhy,
FloresPliego Arturo,
BeltránMontoya Jorge,
ViverosAlcaráz Martín,
VadilloOrtega Felipe
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12179
Subject(s) - term (time) , induction of labor , labor induction , preterm labor , term pregnancy , pregnancy , medicine , obstetrics , biology , gestation , physics , genetics , quantum mechanics
Problem Human parturition is associated with an intrauterine pro‐inflammatory environment in the choriodecidua. Evidence that some mediators of this signaling cascade also elicit responses leading to labor prompted us to characterize the cellular sources of these mediators in the human choriodecidua. Method of study Leukocyte‐enriched preparations from human choriodecidua ( C h L ) and intervillous placental blood leukocytes ( PL ) were maintained in culture. Secretions of inflammatory cytokines, chemokines, and MMP ‐9 were documented. Leukocyte phenotype of C h L and PL was determined by flow cytometry using specific fluorochrome‐conjugated antibodies. Results and Conclusions Ch L showed a distinct pro‐inflammatory secretion pattern of cytokines and chemokines when compared with PL , including higher amounts of TNF ‐α and IL ‐6, and decreased secretions of IL ‐4 and IL ‐1ra. Ch L also secreted more MIP ‐1α and MCP ‐1 and MMP ‐9 than PL . No significant differences were found in leukocytes subsets between compartments. Based on our findings, we propose that C h L isolated from fetal membranes at term are functionally different from PL and may collaborate to modulate the microenvironment linked to induction and progression of human labor.