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Increased Nuclear Expression of Nuclear Factor Kappa‐B p65 Subunit in the Eutopic Endometrium and Ovarian Endometrioma of Women with Advanced Stage Endometriosis
Author(s) -
Kim Sung Hoon,
Ihm Hyo Jin,
Oh Young Sang,
Chae Hee Dong,
Kim ChungHoon,
Kang Byung Moon
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12161
Subject(s) - endometriosis , immunohistochemistry , endometrium , western blot , proinflammatory cytokine , tumor necrosis factor alpha , andrology , cancer research , protein subunit , medicine , endocrinology , biology , inflammation , gene , biochemistry
Problem We evaluated whether the expression of NF ‐кB p65 subunit is increased in the eutopic endometrium and/or in the ovarian endometrioma of women with advanced stage endometriosis, and ascertained in vitro effects of proinflammatory cytokines on the expression and DNA binding of NF ‐кB p65 subunit in endometrial cells. Method of Study Immunohistochemistry was performed to compare the nuclear NF ‐кB p65 subunit immunoreactivity between women with and without advanced stage endometriosis. The nuclear NF ‐кB p65 subunit expression and DNA binding were also analyzed in endometrial cells treated with tumor necrosis factor‐alpha ( TNF ‐α) or interleukin‐1beta ( IL ‐1β) utilizing Western blot analysis, enzyme‐linked immunosorbent assay, and electrophoretic mobility shift assay. Results The immunoreactivity of the nuclear NF ‐кB p65 subunit was significantly increased in the eutopic endometrium as well as in the ovarian endometrioma of women with endometriosis compared with the controls. In vitro treatment of endometrial cells with TNF ‐α and IL ‐1β led to a significant increase in nuclear NF ‐кB p65 subunit expression and DNA binding. Conclusions The nuclear expression of NF ‐κB p65 is increased in the eutopic endometrium and ovarian endometrioma of women with advanced stage endometriosis, which strongly suggests that NF ‐кB signaling plays a crucial role in the pathogenesis and/or pathophysiology of endometriosis.