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Cutting Edge: IL ‐10‐Producing Regulatory B Cells in Early Human Pregnancy
Author(s) -
Rolle Luise,
Memarzadeh Tehran Maryam,
MorellGarcía Anselm,
Raeva Yanitsa,
Schumacher Anne,
Hartig Roland,
Costa SerbanDan,
Jensen Federico,
Zenclussen Ana Claudia
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12157
Subject(s) - regulatory b cells , pregnancy , cd19 , flow cytometry , interleukin 10 , immune system , gestation , immunology , immune tolerance , abortion , regulatory t cell , andrology , biology , medicine , t cell , il 2 receptor , genetics
Problem The function of IL ‐10 producing regulatory B cells (Breg) during gestation is unknown. Here, we aimed to understand their participation in early pregnancy. Method CD 19 + CD 24 hi CD 27 + B cell frequency, measured by flow cytometry, increased with pregnancy onset but not in the case of spontaneous abortions. Results B cells from non‐pregnant women cultured with serum from normal pregnant women produced higher IL‐10 levels than those cultured with serum from spontaneous abortion patients or autologous serum. CD19 + ‐activated B cells from pregnant women strongly suppressed TNF‐a production by CD4 + T cells when cocultured. We identified hCG as an important factor regulating the number and function of Breg during pregnancy. Conclusions Breg emerge as important players in pregnancy; they suppress undesired immune responses from maternal T cells and are therefore important for tolerance acquisition.