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A Study of Forty‐seven Single Nucleotide Polymorphisms among Recurrent Miscarriage Using Classification and Regression Tree Analysis
Author(s) -
Parveen Farah,
Agrawal Suraksha
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12152
Subject(s) - logistic regression , single nucleotide polymorphism , snp , pregnancy , miscarriage , recurrent miscarriage , biology , gestation , genetics , obstetrics , medicine , gynecology , gene , genotype
Problem Successful pregnancy is the result of multiple genetic and non‐genetic factors. Associations of various SNP s described in this study have not revealed any conclusive results. We have analyzed 47 SNP s using statistical tools like multidimensional regression, classification regression tree, and logistic regression. Method of study Two hundred women with at least three consecutive unexplained spontaneous abortions before 20th week of gestation and 300 control women without any history of recurrent miscarriages ( RM ) were genotyped using PCR , RFLP and sequencing. Results Our results revealed that Leptin 2549 C/A (rs7799039) and TNF ‐α 238 (rs361525) may play an important role in the maintenance of pregnancy. TNF ‐ α 238 may act as a protective SNP and Leptin 2549 C/A as a susceptible marker among women with RM cases. Conclusions Present study demonstrated an association with Leptin 2549C/A (rs7799039) and TNF ‐ α (rs361525) gene polymorphism among RM cases.