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Intrauterine Administration of Peripheral Blood Mononuclear Cells (PBMCs) Improves Endometrial Receptivity in Mice with Embryonic Implantation Dysfunction
Author(s) -
Yu Nan,
Yang Jing,
Guo Yue,
Fang Jianye,
Yin Tailang,
Luo Jing,
Li Xing,
Li Wei,
Zhao Qinghong,
Zou Yujie,
Xu Wangming
Publication year - 2014
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12150
Subject(s) - peripheral blood mononuclear cell , implantation failure , embryo , medicine , andrology , pregnancy , endometrium , pregnancy rate , uterus , embryo transfer , uterine horns , immunohistochemistry , biology , in vitro , infertility , genetics , microbiology and biotechnology , biochemistry
Objective Intrauterine administration of autologous peripheral blood mononuclear cells ( PBMC s) activated by HCG in vitro is reported to improve implantation rates in patients with repeated failure of IVF ‐ ET . In this article, the impact of intrauterine administration of PBMC s on embryo implantation, pregnancy rate and the underlying mechanisms will be investigated. Methods Pregnant mice were randomly divided into three groups, including control group; embryo implantation dysfunction ( EID ) group; EID with PBMC s group. Uterine horns were excised to determine the number of pregnant mice and implantation sites on the Day 7.5 postcoitum. The expression levels of LIF and VEGF during the implantation window were detected with immunohistochemistry and Real Time‐ PCR analysis. Results Embryo implantation dysfunction model group showed a significant decrease in pregnancy rate, implantation sites and the expression of both the endometrial LIF and VEGF during the implantation window. EID with PBMCs group showed a higher pregnancy rate and endometrial LIF and VEGF expression compared to EID group. Conclusion Intrauterine administration of mouse PBMC s derived from unpregnant mice prior to embryo implant has a good influence on endometrial receptivity and embryonic implantation in EID mice.