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The Gene Expression Analysis of Paracrine/Autocrine Factors in Patients with Spermatogenetic Failure Compared with Normal Spermatogenesis
Author(s) -
Malcher Agnieszka,
Rozwadowska Natalia,
Stokowy Tomasz,
Jedrzejczak Piotr,
Zietkowiak Wojmir,
Kurpisz Maciej
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12149
Subject(s) - autocrine signalling , paracrine signalling , spermatogenesis , azoospermia , biology , andrology , microarray , gene expression , sertoli cell , microarray analysis techniques , gene , male infertility , medicine , endocrinology , infertility , genetics , cell culture , pregnancy , receptor
Problem The aim of this study was to examine the expression levels of IL ‐1 family members, IL ‐6, IL ‐10, TNF family, SCF , and c‐ kit in infertile patients with idiopathic non‐obstructive azoospermia ( NOA ) compared with men with normal spermatogenesis. Method of study We analyzed 20 testicular biopsy samples with Affymetrix Human Gene 1.0 ST microarrays (Affymetrix, Santa Clara, CA, USA). Sixteen of them were obtained from patients with various types of NOA and four with normal spermatogenesis. Results The comparative analysis of normal and pathological group demonstrated a different expression level of IL 1‐ RA gene. It was also observed that the gene expression levels for IL 1‐R1, CASP 1, and stem cell factor ( SCF ) were upregulated in the Sertoli‐cell‐only syndrome group in comparison with the control one ( P < 0.05). Conclusion The microarray analysis showed the expression level of all investigated paracrine/autocrine factors at one go, and therefore, the possible interaction between these genes could be examined.