Premium
HSP 90 Antibodies: A Detrimental Factor Responsible for Ovarian Dysfunction
Author(s) -
Choudhury Asmita,
Khole Vrinda V.
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12136
Subject(s) - folliculogenesis , heat shock protein , biology , autoantibody , antibody , ovary , hsp90 , endocrinology , infertility , medicine , andrology , oogenesis , immunology , embryo , cryopreservation , microbiology and biotechnology , gene , pregnancy , biochemistry , genetics
Problem Earlier studies from our group have established that about 47% cases of autoimmune ovarian failure are due to presence of autoantibodies to H eat S hock P rotein 90 ( HSP 90). However, there are no reports correlating pathological effects of HSP 90 autoantibodies leading to ovarian failure. Method of study Antibodies to HSP 90 in female mouse model were generated by active immunization with an immunodominant peptide of HSP 90, followed by detailed analysis of several reproductive parameters. Result Estrous cyclicity remains unchanged; however, there was a significant drop in the fertility index due to an increase in pre‐ and post‐implantation loss, associated with an increased incidence of degenerated eggs and embryos. The ovaries showed an increase in the number of empty and degenerated follicles and extensive granulosa cell deaths, which was reflected by the decrease in the levels of N obox and G ja1 gene expression. Conclusion This study underlines a critical role played by HSP 90 in ovarian folliculogenesis and highlights the implications of the presence of anti‐ HSP 90 antibodies in infertile women.