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Blocking of Stromal Cell–Derived Factor‐1 Reduces Neoangiogenesis in Human Endometriosis Lesions in a Mouse Model
Author(s) -
Virani Sophia,
Edwards Andrew K.,
Thomas Richard,
Childs Timothy,
Tayade Chandrakant
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12134
Subject(s) - stromal cell , cd34 , endometriosis , neovascularization , endometrium , angiogenesis , cd31 , progenitor cell , medicine , vascular endothelial growth factor , endothelial progenitor cell , kinase insert domain receptor , cancer research , pathology , biology , stem cell , vascular endothelial growth factor a , vegf receptors , genetics
Problem Endometriosis affects 5–10% of women and is characterized by the growth of endometrial tissue outside of the uterus. Establishing new blood supply is a fundamental requirement for endometriosis lesion growth. Endothelial progenitor cells ( EPC s), recruited by stromal cell–derived factor‐1 ( SDF ‐1), contribute to neoangiogenesis in endometriotic lesions. We hypothesized that SDF ‐1 is central to the neoangiogenesis and survival of endometriotic lesions, and blocking of SDF ‐1 will reduce vascularization of lesions in a mouse model. Method of study Using immunohistochemistry, we evaluated SDF ‐1 and CD 34 + EPC s in human endometriotic lesions and normal endometrium samples. EPC s were co‐localized using CD 34 and VEGFR 2. Effects of SDF ‐1 blocking on endometriotic lesion survival were assessed in BALB /c‐Rag2 −/− / IL 2rγ −/− mice engrafted with human endometrium and treated with SDF ‐1‐blocking antibody or an isotype control. Weekly blood samples from experimental mice were analyzed for cytokines and EPC s. Results SDF ‐1 and CD 34 + EPC s were abundant in human endometriotic lesions compared with eutopic endometrium. In our mouse model, SDF ‐1‐blocking antibody reduced CD 31 + microvessels compared with isotype control. Conclusion Blocking SDF ‐1 reduces neovascularization and survival of lesions in a mouse model of endometriosis.