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Human Endometrial Endothelial Cells Generate Distinct Inflammatory and Antiviral Responses to the TLR 3 agonist, Poly(I:C) and the TLR 8 agonist, viral ss RNA
Author(s) -
Krikun Graciela,
Potter Julie A.,
Abrahams Vikki M.
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12128
Subject(s) - agonist , tlr3 , inflammation , biology , virology , chemistry , immunology , receptor , medicine , innate immune system , immune system , toll like receptor
Problem Human endometrial endothelial cell ( HEEC ) innate immunity remains poorly characterized. Based on their direct contact with the circulation, HEEC s are uniquely positioned to be exposed to viral infections. This study evaluated the innate immune response generated by HEEC s after exposure to the TLR 3 agonist, Poly(I:C) and the TLR 8 agonist, viral ss RNA . Method of Study HEECs were treated with or without P oly(I:C) or ss RNA . Culture supernatants were measured for cytokines by multiplex analysis. RNA was analyzed by qRT ‐ PCR for type I interferons and antiviral factors. Results Treatment of HEEC s with Poly(I:C) rapidly upregulated the secretion of IL‐2, IL‐6, IL‐8, IFN ‐γ, G ‐ CSF , GM ‐ CSF , MCP ‐1, MIP ‐1β, RANTES , and GRO ‐α after 12 hr, while ss RNA treatment induced the slower secretion of IL‐6, IL‐8, IFN‐γ, G ‐ CSF , VEGF , and GRO ‐α after 24 hr. Both viral components induced HEEC IFN‐α and IFN‐β expression. While treatment with Poly(I:C) induced APOBEC3G and OAS expression, treatment with ssRNA upregulated APOBEC 3 G and M×A mRNA . Conclusion Our findings demonstrate that HEEC s can differentially sense and respond to viral components by generating distinct inflammatory and antiviral immune responses, indicating that these cells likely play an active role in the immune protection of the uterus toward viral infections.