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CD40, CD80, and CD86 Costimulatory Molecules are Differentially Expressed on Murine Splenic Antigen‐presenting Cells During the Pre‐implantation Period of Pregnancy, and they Modulate Regulatory T‐cell Abundance, Peripheral Cytokine Response, and Pregnancy Outcome
Author(s) -
Slawek Anna,
Maj Tomasz,
ChelmonskaSoyta Anna
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12108
Subject(s) - cd80 , spleen , cd86 , biology , immunology , immune system , cytokine , flow cytometry , antibody , t cell , antigen , regulatory t cell , foxp3 , cd40 , andrology , medicine , endocrinology , cytotoxic t cell , il 2 receptor , in vitro , biochemistry
Problem The object of the study was to investigate the costimulatory phenotype of spleen antigen‐presenting cells ( APC s) in mice after mating and the influence of costimulatory signal blocking on pregnancy outcome, cytokine production, and Treg cell concentration. Method of study The levels of CD 40, CD 80, and CD 86 on spleen APC s at day 0.5 and 3.5 after mating (C57 BL /6Jx DBA /2J and C57 BL /6JxBalb/c) were assessed by flow cytometry and RT ‐ PCR . Blocking antibodies against costimulatory molecules were given i.p. at day 3.5 after mating. Pregnancy outcomes, blood cytokine ( ELISA ), and spleen Treg (flow cytometry) concentrations were examined at day 10.5 after mating. Results Differential expression of costimulatory molecules on spleen APC s of mated v. pseudopregnant mice was observed mainly at day 3.5 after conception. Administration of anti‐ CD 86 antibody lowered pregnancy outcome. Cytokine expression was modulated after administration of anti‐ CD 86, anti‐ CD 80 and anti‐ CD 40 antibodies, whereas only anti‐ CD 40 antibody changed the concentration of Treg lymphocytes and the level of Foxp3 protein expression. Conclusion Costimulatory phenotype of female spleen APC s is distinctly modulated after mating. Alteration of costimulatory signal derived from APC s during pre‐implantation period of pregnancy may have an adverse effect on pregnancy outcome and the tolerogenic immune response.