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Cyclic Stretch Augments Production of Neutrophil Chemokines and Matrix Metalloproteinases‐1 ( MMP ‐1) from Human Decidual Cells, and the Production was Reduced by Progesterone
Author(s) -
Zhao Yin,
Koga Kaori,
Osuga Yutaka,
Izumi Gentaro,
Takamura Masashi,
Harada Miyuki,
Hirata Tetsuya,
Hirota Yasushi,
Yoshino Osamu,
Inoue Satoshi,
Fujii Tomoyuki,
Kozuma Shiro
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12092
Subject(s) - chemotaxis , chemokine , matrix metalloproteinase , decidual cells , microbiology and biotechnology , decidua , chemistry , interleukin 8 , medicine , endocrinology , andrology , biology , immunology , inflammation , receptor , biochemistry , placenta , pregnancy , fetus , genetics
Problem The purpose of this study was to evaluate the impact of mechanical stretch caused by uterine contraction and progesterone (P 4 ) on decidual cells ( DC ), neutrophil chemokines, and MMP ‐1 expression. Method of study DC were cultured, and cyclic stretch was applied using a computer‐operated cell stretch system. Interleukin (IL)‐8, growth‐regulated oncogene (GRO) α, matrix metalloproteinase (MMP)‐1, and mRNA and/or protein expression/activity was measured using RT‐PCR and ELISA. Neutrophil chemotactic activity in conditioned media was evaluated using migration assays. The effect of P 4 was also studied. Results Cyclic mechanical stretch increased IL‐8, GROα, mRNA and protein, and MMP‐1 production and activity level. Supernatant from stretched cells induced neutrophil chemotactic activity significantly. P 4 suppressed the effect of stretch. Conclusion The current study demonstrates that cyclic mechanical stretch stimulates the production of neutrophil chemokines and MMP ‐1 from human decidual cells, and the production was reduced by progesterone. These findings suggest that decidual cells are responding to mechanical and endocrine signals and induce biochemical factors and thereby contribute to the regulation of human labor.