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B7 Costimulation and Intracellular Indoleamine‐2,3‐Dioxygenase Expression in Peripheral Blood of Healthy Pregnant and Pre‐Eclamptic Women
Author(s) -
Toldi Gergely,
Vásárhelyi Barna,
Biró Enikő,
Fügedi Gergely,
Rigó János,
Molvarec Attila
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12069
Subject(s) - indoleamine 2,3 dioxygenase , immunology , peripheral blood , flow cytometry , peripheral tolerance , pathogenesis , immune tolerance , intracellular , immune system , peripheral , medicine , pregnancy , inflammation , biology , microbiology and biotechnology , tryptophan , biochemistry , amino acid , genetics
Problem We determined the frequency of activated ( CD 11b+) monocytes expressing B7‐1, B7‐2, B7‐H1, and B‐7H2, and that of T cells and T helper cells expressing CD 28, CTLA ‐4, PD ‐1, and ICOS in peripheral blood samples from normal pregnant ( NP ) and pre‐eclamptic ( PE ) women. We also examined the intracellular expression of indoleamine‐2,3‐dioxygenase ( IDO ). Method of study We measured the expression of the above markers using flow‐cytometry in peripheral blood samples from 20 NP and 20 PE women in the third trimester. Results The frequency of B7‐1 and B7‐2 expressing activated monocytes and that of IDO expressing T‐lymphocytes was lower in PE than in NP . Conclusion Lower expression of B7‐1 and B7‐2 proteins on peripheral monocytes in PE might indicate a secondary regulatory mechanism in response to the ongoing systemic maternal inflammation. IDO plays an important role in the pregnancy‐specific immune tolerance, and might be a contributing factor in the pathogenesis of PE .