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Relationship Between Toll‐like Receptor‐4 and mPGES ‐1 Gene Expression in Local Lesions of Endometriosis Patients
Author(s) -
Hayashi Chuyu,
Chishima Fumihisa,
Sugitani Masahiko,
Ichikawa Go,
NakazawaWatanabe Teine,
Sugita Kenji,
Suzuki Manami,
Nemoto Norimichi,
Yamamoto Tatsuo
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12056
Subject(s) - endometriosis , innate immune system , immunohistochemistry , pathogenesis , receptor , messenger rna , endometrium , immune system , toll like receptor , biology , medicine , cancer research , immunology , gene , biochemistry
Problem Toll‐like receptors (TLRs) are innate immune receptors that mediate the pattern recognition of, and response toward, pathogens and host‐derived danger signals. We reported that cyclooxygenase‐2 (COX‐2) and microsomal prostaglandin E synthase ( mPGES ) mRNA were expressed in cases of endometriosis. The relationship between COX ‐2, mPGES ‐1, and TLR 4 in endometriotic lesions has yet to be determined. Method of study Endometriosis samples were obtained from 37 patients with endometrial cysts. Endometrial tissues were obtained from patients undergoing surgical procedures for benign gynecological conditions. COX ‐2, mPGES ‐1, and TLR 4 mRNA expressions were examined by real‐time quantitative reverse transcription PCR ( qRT ‐PCR) and mPGES ‐1, and TLR 4 protein localization was examined by immunohistochemistry. Results TLR4 proteins were mostly located to the glandular epithelium. The immunoreactivities of TLR 4 and mPGES ‐1 from endometriosis lesions were significantly higher than those in eutopic endometrium in the proliferative phase. The expression levels of mPGES ‐1 mRNA in peritoneal endometriosis were higher than those in eutopic endometrium in the proliferative phase. The expression of TLR 4 mRNA correlates with that of mPGES ‐1 mRNA and not with that of COX ‐2 in endometriotic lesions. Conclusion Relationship between TLR 4 and mPGES ‐1 mRNA in endometriotic lesions indicate that innate immunity may play an important role in the pathogenesis of endometriosis.