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Effect of Carbon Monoxide on Bacteria‐Stimulated Cytokine Production by Placental Explants
Author(s) -
Peltier Morgan R.,
Arita Yuko,
Gurzenda Ellen M.,
Klimova Natalia,
Koo HschiChi,
Murthy Amitasrigowri,
Hanzeeh
Publication year - 2013
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12017
Subject(s) - proinflammatory cytokine , cytokine , prostaglandin e2 , microbiology and biotechnology , biology , tumor necrosis factor alpha , bacteria , andrology , prostaglandin e , placenta , explant culture , chemistry , immunology , inflammation , endocrinology , medicine , biochemistry , in vitro , pregnancy , fetus , genetics
Problem Preterm birth is frequently caused by an inflammatory response to ascending infections of the reproductive tract. Carbon monoxide ( CO ) has potent anti‐inflammatory properties at subtoxic concentrations. Whether or not CO can modulate inflammatory responses by placental tissues is unclear. Methods Placental explant cultures were incubated with heat‐killed E scherichia coli or U reaplasma parvum in the presence or absence of 250 ppm CO for 24 hr. Concentrations of cytokines relative viability of the cultures were quantified. Results E scherichia coli‐ and U . parvum ‐stimulated IL ‐1β production was significantly inhibited by CO supplementation. E scherichia coli‐stimulated , but not U . parvum ‐stimulated, IFN ‐γ production was inhibited by CO . While CO inhibited PGE 2 production by unstimulated cells, no effects on bacteria‐stimulated prostaglandin production were detected. CO had no effect on basal or E . coli ‐stimulated TNF ‐α production but enhanced TNF‐α production by cultures stimulated with U . parvum . In addition, CO tended to improve the viability of the placental cultures. Conclusions Low concentrations of CO tended to reduce proinflammatory cytokines and to promote the production of anti‐inflammatory cytokines in a pathogen‐specific manner. These properties suggest that CO may be useful for promoting a pro‐pregnancy cytokine milieu by placental explants and may reduce the consequences of intrauterine infections.