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Analysis of HLA‐G Polymorphisms in Couples with Implantation Failure
Author(s) -
Nardi Fabiola da Silva,
Slowik Renata,
Wowk Pryscilla Fanini,
da Silva José Samuel,
Gelmini Geórgia Fernanda,
Michelon Tatiana Ferreira,
Neumann Jorge,
Bicalho Maria da Graça
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/aji.12001
Subject(s) - allele , hla g , human leukocyte antigen , embryo , biology , immune system , exon , single nucleotide polymorphism , genotype , immunology , pregnancy , typing , andrology , genetics , gene , medicine , antigen
Problem HLA ‐ G expression is related as an immune modulator of fetal–maternal tolerance, and its levels was correlated with pregnancy outcome. In a case–control study, we investigate the association between the genetic variability of the HLA‐G gene and serum levels of soluble HLA ‐G in cases of embryo implantation failure. Method of Study Forty couples with at least two unsuccessful fresh embryo transfers (implantation failure; IF ) and 83 fertile couples with at least two successful pregnancies was genotyped by sequencing‐based typing. HLA ‐ G alleles were defined by nucleotide sequence variations at exon 2, 3, and 4, and the quantification of soluble HLA ‐ G (s HLA ‐ G ) was performed by ELISA . Results There was a significant difference between the HLA ‐ G allelic distributions between IF couples and the control couples. The HLA‐G*01:03:01 allele was increased in the IF couples. There were no significant differences in the serum levels of s HLA ‐ G in the IF and control groups. Conclusion The results suggest that the distribution of HLA ‐ G products may play a significant role in the modulation of maternal–fetal immune response.