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Probiotic mixture reduces gut inflammation and microbial dysbiosis in children with atopic dermatitis
Author(s) -
Yoon Wonsuck,
Park Sang Hyun,
Lee Jue Seong,
Byeon Jung Hye,
Kim Seung Hyun,
Lim Jaehoon,
Yoo Young
Publication year - 2021
Publication title -
australasian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.67
H-Index - 53
eISSN - 1440-0960
pISSN - 0004-8380
DOI - 10.1111/ajd.13644
Subject(s) - probiotic , dysbiosis , calprotectin , atopic dermatitis , medicine , microbiome , scorad , gut flora , gastroenterology , faecal calprotectin , immunology , bifidobacterium , food science , biology , lactobacillus , inflammatory bowel disease , bacteria , psoriasis , bioinformatics , genetics , disease , dermatology life quality index , fermentation
Background Recent data suggested that dysbiosis of the gut microbiome is associated with childhood allergic diseases. Oral administration of probiotic formulations may improve the severity of atopic dermatitis (AD) by restoring imbalanced gut microbiota and reducing intestinal inflammation in children. Objectives The aim of this study was to investigate the effects of a probiotic mixture on the clinical severity of AD, gut inflammatory markers and alterations in microbiome dysbiosis in children with AD. Methods A total of 25 subjects were enrolled in this study and administered with a mixture of probiotic strains consisting of Lactobacilli and Bifidobacteria for 4 weeks. The clinical efficacy of the probiotic mixture was assessed using SCORAD index and TEWL. Faecal calprotectin levels were measured as a marker for intestinal inflammation. The composition and diversity of the gut microbiome were analysed using 16S rRNA pyrosequencing. Results The SCORAD (38.9 ± 17.2 vs 29.0 ± 15.4, P < 0.001) and TEWL (58.3 ± 12.5 vs 27.3 ± 8.7 g/m 2 /h, P = 0.028) were significantly decreased after 4 weeks administration of the probiotic mixture. The faecal calprotectin level (121.5 [27.7–292.9] vs 37.0 μg/g [12.6–108.9 μg/g], P = 0.038) was significantly decreased. The α‐diversity and composition of the gut microbiome were not significantly changed, but β‐diversity was increased after 4 weeks. Conclusions The oral administration of the probiotic mixture was effective in reducing clinical severity and intestinal inflammation in children with AD. Gut microbial diversity was slightly increased after administration of the probiotic mixture. The results of this study suggest that a probiotic mixture can alleviate AD by decreasing inflammation and modulating the gut microbiota in children with AD.