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Protective effect of skin‐derived precursors on photoaging in nude mice
Author(s) -
Wang Siyu,
Zhong Jianqiao,
Li Li
Publication year - 2019
Publication title -
australasian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.67
H-Index - 53
eISSN - 1440-0960
pISSN - 0004-8380
DOI - 10.1111/ajd.12867
Subject(s) - photoaging , transplantation , dermis , medicine , in vivo , immunohistochemistry , andrology , dermatology , pathology , cancer research , microbiology and biotechnology , biology
Objectives Currently, innovative methods to prevent photoaging are needed. Skin‐derived precursors ( SKP ) have been shown to play a crucial role in resisting UVB ‐induced apoptosis in vitro . The objective of this study was to explore the effect of SKP on preventing skin photoaging in vivo . Methods Skin‐derived precursors from neonatal BALB /c mice were isolated, identified and intradermally transplanted with a PKH 26 label to track their survival. These were then injected at different concentrations into the buttock dermis of nude mice at 2‐weekly intervals before UV irradiation. Photographs, assessment of live skin surface, histology with quantitative real‐time polymerase chain reaction and immunohistochemistry were used to evaluate the impact of SKP on wrinkles and other relevant indicators of skin photoaging. Results SKP exhibited a sphere‐like structure and could survive for at least 2 weeks after intradermal transplantation. A large dose of SKP transplantation (10 5 SKP + UV ) at 2‐weekly intervals were able to ameliorate coarse UV ‐induced wrinkles. Moreover, the skin smoothness value, dermal thickness and collagen percentage were significantly increased in mice that received a large dose of SKP (10 5 SKP + UV ). UV radiation induced the mRNA expression of MMP ‐13 and decreased the mRNA and protein expression of Tβ RII , but these effects were diminished by SKP transplantation. The transplantation of SKP could increase the mRNA of TIMP ‐1 . Conclusions We found that transplanted SKP exert a beneficial impact on preventing UV ‐induced wrinkles in vivo , suggesting that SKP transplantation is a promising candidate for preventing photoaging.