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Upregulated expression of La ribonucleoprotein domain family member 6 and collagen type I gene following water‐filtered broad‐spectrum near‐infrared irradiation in a 3‐dimensional human epidermal tissue culture model as revealed by microarray analysis
Author(s) -
Tanaka Yohei,
Nakayama Jun
Publication year - 2018
Publication title -
australasian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.67
H-Index - 53
eISSN - 1440-0960
pISSN - 0004-8380
DOI - 10.1111/ajd.12604
Subject(s) - downregulation and upregulation , gene expression , microbiology and biotechnology , irradiation , gene , human skin , real time polymerase chain reaction , microarray analysis techniques , infrared , biology , chemistry , pathology , medicine , genetics , optics , physics , nuclear physics
Background/Objectives Water‐filtered broad‐spectrum near‐infrared irradiation can induce various biological effects, as our previous clinical, histological, and biochemical investigations have shown. However, few studies that examined the changes thus induced in gene expression. The aim was to investigate the changes in gene expression in a 3‐dimensional reconstructed epidermal tissue culture exposed to water‐filtered broad‐spectrum near‐infrared irradiation. Methods DNA microarray and quantitative real‐time polymerase chain reaction ( PCR ) analysis was used to assess gene expression levels in a 3‐dimensional reconstructed epidermal model composed of normal human epidermal cells exposed to water‐filtered broad‐spectrum near‐infrared irradiation. The water filter allowed 1000–1800 nm wavelengths and excluded 1400–1500 nm wavelengths, and cells were exposed to 5 or 10 rounds of near‐infrared irradiation at 10 J/cm 2 . Results A DNA microarray with over 50 000 different probes showed 18 genes that were upregulated or downregulated by at least twofold after irradiation. Quantitative real‐time PCR revealed that, relative to control cells, the gene encoding La ribonucleoprotein domain family member 6 ( LARP 6 ), which regulates collagen expression, was significantly and dose‐dependently upregulated ( P < 0.05) by water‐filtered broad‐spectrum near‐infrared exposure. Gene encoding transcripts of collagen type I were significantly upregulated compared with controls ( P < 0.05). Conclusions This study demonstrates the ability of water‐filtered broad‐spectrum near‐infrared irradiation to stimulate the production of type I collagen.