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Significance of CEP78 and WDR62 gene expressions in differentiated thyroid carcinoma: Possible predictors of lateral lymph node metastasis
Author(s) -
Hammad Maha O.,
Elabbasy Lamiaa M.,
Abd elghaffar Mahmoud A.,
Zaki Marwa M. A.,
Bazeed Fagr B.,
Zahran Mohammed A.
Publication year - 2019
Publication title -
asia‐pacific journal of clinical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 29
eISSN - 1743-7563
pISSN - 1743-7555
DOI - 10.1111/ajco.13143
Subject(s) - medicine , goiter , thyroid cancer , thyroid carcinoma , clinical significance , metastasis , pathological , lymph node metastasis , thyroid , pathology , biomarker , confidence interval , real time polymerase chain reaction , oncology , cancer , biology , gene , biochemistry
Objectives This study aimed at investigating the clinical significance of CEP78 and WDR62 in differentiated thyroid carcinoma (DTC). This study also aimed at finding predictors that help in detecting patients with DTC who have high risk for lateral lymph node metastasis (LNM). Methods Quantitative real‐time polymerase chain reaction (RT‐qPCR) was performed to examine CEP78, and WDR62 mRNA expressions in 40 tissue specimens of DTC, and 40 goiter tissue specimens. Additionally, we reviewed clinical, ultrasound, laboratory, pathological data of patients to analyze the associations between these characteristics and lateral LNM. Results Our results demonstrated that relative CEP78 mRNA levels were significantly decreased in thyroid cancer tissues than goiter tissues ( P = 0.002). ROC curve analysis confirmed the diagnostic value of CEP78 mRNA expression, providing an AUC equals to 0.698 (95% confidence intervals (CI), 0.583–0.813; P = 0.002). The relative WDR62 mRNA expression was not statistically different in DTC tissues and goiter tissues ( P = 0.686). Furthermore, the DTC patients had been included to examine risk factors for lateral LNM. In multivariate analysis, the significant factors for predicting lateral LNM were low CEP78 mRNA expression (cut off value ≤0.54; P = 0.03; OR = 19.62; 95% CI, 1.3–296.23), central LNM ( P = 0.011; OR = 33.6; 95% CI, 2.24–503.6) and calcifications ( P = 0.023; OR = 27.187; 95% CI, 1.57–469.5). Conclusions CEP78 can be used as a promising molecular biomarker for differentiation between DTC and goiter tissues, in addition it might serve as a predictor of lateral LNM in DTC along with central LNM and calcifications. Unlike CEP78, WDR62 mRNA expression was not statistically different in DTC and goiter.