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Downregulated miR‐17, miR‐29c, miR‐92a and miR‐214 may be related to BCL11B overexpression in T cell acute lymphoblastic leukemia
Author(s) -
He Zifan,
Liao Ziwei,
Chen Shaohua,
Li Bo,
Yu Zhi,
Luo Gengxin,
Yang Lijian,
Zeng Chengwu,
Li Yangqiu
Publication year - 2018
Publication title -
asia‐pacific journal of clinical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 29
eISSN - 1743-7563
pISSN - 1743-7555
DOI - 10.1111/ajco.12979
Subject(s) - microrna , downregulation and upregulation , peripheral blood mononuclear cell , cell growth , leukemia , cancer research , biology , apoptosis , microbiology and biotechnology , immunology , in vitro , gene , genetics
Aim BCL11B overexpression is a characteristic of most T cell acute lymphoblastic leukemia (T‐ALL) cases, and downregulated BCL11B in leukemic T cells inhibits cell proliferation and induces apoptosis. The purpose of this study was to analyze the miRNA expression pattern that may be related to BCL11B regulation in T‐ALL. Methods Quantitative real‐time PCR was used to detect the miRNAs miR‐17‐3p, miR‐17‐5p, miR‐29c‐3p, miR‐92a‐3p, miR‐214‐3p and miR‐214‐5p, the BCL11B expression level in peripheral blood mononuclear cells which was obtained from 17 de novo and untreated T‐ALL patients, and 15 healthy individuals (HIs) served as control. Correlations between the relative miRNA expression levels and BCL11B were analyzed. Results Based on the computational prediction that certain miRNAs bind the BCL11B 3′‐UTR, miR‐17‐3p, miR‐17‐5p, miR‐29c‐3p, miR‐92a‐3p, miR‐214‐3p and miR‐214‐5p were found to be candidates for regulating BCL11B . The expression levels of the six miRNAs were decreased compared with HIs, and with the exception of miR‐17‐5p, statistically significant differences in expression levels were found in the T‐ALL group. Moreover, while significantly higher BCL11B expression was found in the T‐ALL group, a negative trend in the correlation level for all six miRNAs could be found in all groups; however, statistical significance was only found for miR‐214‐3p in the T‐ALL group. Conclusion miRNA downregulation together with BCL11B upregulation suggests that miR‐17, miR‐29c, miR‐92a and miR‐214 might be involved in BCL11B regulation. The therapeutic promise of regulating the expression of these miRNAs for T‐ALL therapy may be considered in the future.