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Spectrum of EGFR gene mutations in Vietnamese patients with non–small cell lung cancer
Author(s) -
Vu Hoang Anh,
Xinh Phan Thi,
Ha Hua Thi Ngoc,
Hanh Ngo Thi Tuyet,
Bach Nguyen Duc,
Thao Doan Thi Phuong,
Dat Ngo Quoc,
Trung Nguyen Sao
Publication year - 2016
Publication title -
asia‐pacific journal of clinical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 29
eISSN - 1743-7563
pISSN - 1743-7555
DOI - 10.1111/ajco.12448
Subject(s) - sanger sequencing , exon , epidermal growth factor receptor , lung cancer , biology , mutation , t790m , cancer research , gene mutation , gene , dna sequencing , microbiology and biotechnology , cancer , oncology , medicine , genetics , gefitinib
Aim Epidermal growth factor receptor ( EGFR ) mutational status is a crucial biomarker for prediction of response to tyrosine kinase inhibitors in patients with non–small cell lung cancer (NSCLC). Although these mutations have been well characterized in other countries, little is known about the frequency or spectrum of EGFR mutations in Vietnamese NSCLC patients. Methods Using Sanger DNA sequencing, we investigated mutations in EGFR exons 18–21 from 332 patients diagnosed with NSCLC at University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam. DNA was extracted from formalin‐fixed, paraffin‐embedded tissues, followed by PCR amplification and sequencing. Results EGFR mutations were detected in 135 samples (40.7%), of which eight samples carried double mutations. In total, 46 different types of EGFR mutations were found, including six novel mutations (p.K713E, p.K714R, p.P794S, p.R803W, p.P848S, and p.K867E). Among the four exons investigated, exon 19 was most frequently mutated (63 out of 332 patients, 19%), with the p.E746_A750del appearing in 43 samples. Exon 21 was mutated in 56 samples (16.9%), of which 47 were p.L858R. Each of exons 18 and 20 was mutated in 12 samples (3.6%). The frequency of EGFR mutations was higher in females than in males (48.9% vs 35%, P = 0.012), but not statistically different between adenocarcinomas and other histological types of NSCLC (41.3% vs 34.5%, P = 0.478). Conclusion DNA sequencing detected EGFR mutations with high frequency and revealed a broad spectrum of mutation type in Vietnamese patients with NSCLC.