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Trade‐off expression of melatonin receptor subtypes (Mel1a and Mel1b) and androgen receptor in lung of a tropical bird, Perdicula asiatica
Author(s) -
Kharwar Rajesh Kumar,
Singh Vaishali,
Haldar Chandana
Publication year - 2020
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12548
Subject(s) - melatonin , androgen receptor , melatonin receptor , testosterone (patch) , endocrinology , medicine , androgen , receptor , biology , hormone , downregulation and upregulation , steroid hormone , prostate cancer , biochemistry , cancer , gene
The role of circulatory steroid hormone along with melatonin in lung of any seasonally breeding bird has never been explored so far. This could be interesting because steroid hormones are immunosuppressive while melatonin is immunostimulatory in nature. In our present study, we report the effect of exogenous melatonin and testosterone on expression of melatonin receptor subtypes (Mel 1a and Mel 1b ) and androgen receptor in lung of a tropical bird Perdicula asiatica . Birds were collected from vicinity of Varanasi and acclimatized in laboratory with sufficient food and water. The birds were treated with melatonin and testosterone at dose of 25 µg/100 g B.wt./day and 1 mg/100 g B.wt./day, respectively, for 28 days. At the end of the experiment, the birds were sacrificed and lung tissue and blood sample were collected for immunohistochemistry, Western blot analysis and hormonal assay. Testosterone treatment increased circulatory testosterone and upregulated expression of androgen receptors whereas downregulated expression of melatonin receptor subtypes Mel 1a and Mel 1b . Melatonin administration increased peripheral melatonin and upregulated expression of melatonin receptor subtypes Mel 1a and Mel 1b while downregulated androgen receptor. Thus, our results suggest that a trade‐off relationship between melatonin and testosterone exists in regulation of their receptors in lung of Perdicula asiatica .