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Lipid droplets in clusters negatively affect Bos indicus embryos during cryopreservation
Author(s) -
LópezDamián Eva Patricia,
JiménezMedina José Alfredo,
Lammoglia Miguel Angel,
Pimentel Jaime Arturo,
AgredanoMoreno Lourdes Teresa,
Wood Chris,
Galina Carlos Salvador,
Fiordelisio Tatiana
Publication year - 2018
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12382
Subject(s) - cryopreservation , embryo , lipid droplet , biology , sudan black b , andrology , chemistry , zoology , biochemistry , microbiology and biotechnology , genetics , staining , medicine
Embryo transfer using cryopreserved B. indicus embryos results in low pregnancy rates. The low viability of B. indicus embryos is likely a result of their low freezability relative to embryos from other species, notably B. taurus . Freezability is probably related to the quantity of lipid droplets in the embryonic cells. However, the mechanism underlying this association is unknown. Using multiphoton and transmission electron microscopy, the proportion, volume, morphology and composition of lipid droplets in fresh and cryopreserved B. indicus and B. taurus embryos were studied. B. indicus embryos have more droplets than B. taurus and a greater percentage of lipid droplets in clusters (34% vs. 24%; p < 0.05). Also, B. indicus individual droplets are of greater volume than those of B. taurus (47 μm 3 vs. 34 μm 3 ; p < 0.05 ) . After freezing, the percentage of clusters decreased in B. indicus (11% vs. 5%; p < 0.05), while in B. taurus they increased (2% vs. 7%; p < 0.05). Freezing influenced the lipid droplet morphology, increasing the number of cracked droplets in B. indicus embryos (33% fresh vs. 62% frozen ; p < 0.05), but not in B. taurus embryos ( 35% fresh vs. 34% frozen; ns) . Regarding composition, saturated lipid droplets prevail in B. indicus embryos , contrary to B. taurus embryos. We observed differences in lipid droplet organization, proportion, volume, morphology and composition between B. indicus and B. taurus embryos which are altered in opposite directions following freezing.