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Heterogeneous expression of glycoconjugates in the primary olfactory centre of the Japanese sword‐tailed newt ( Cynops ensicauda )
Author(s) -
Matsui T.,
Tanaka K.,
Kobayashi Y.
Publication year - 2018
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12320
Subject(s) - vomeronasal organ , olfactory bulb , olfactory system , biology , glycoconjugate , wheat germ agglutinin , microbiology and biotechnology , lectin , anatomy , neuroscience , central nervous system , biochemistry
Summary Histochemical organization of the Caudata olfactory system remains largely unknown, despite this amphibian order showing phylogenetic diversity in the development of the vomeronasal organ and its primary centre, the accessory olfactory bulb. Here, we investigated the glycoconjugate distribution in the olfactory bulb of a semi‐aquatic salamander, the Japanese sword‐tailed newt ( Cynops ensicauda ), by histochemical analysis of the lectins that were present. Eleven lectins showed a specific binding to the olfactory and vomeronasal nerves as well as to the olfactory glomeruli. Among them, succinylated wheat germ agglutinin (s‐ WGA ), soya bean agglutinin ( SBA ), Bandeiraea simplicifolia lectin‐I ( BSL ‐I) and peanut agglutinin showed significantly different bindings to glomeruli between the main and accessory olfactory bulbs. We also found that s‐ WGA , SBA , BSL ‐I and Pisum sativum agglutinin preferentially bound to a rostral cluster of glomeruli in the main olfactory bulb. This finding suggests the presence of a functional subset of primary projections to the main olfactory system. Our results therefore demonstrated a region‐specific glycoconjugate expression in the olfactory bulb of C. ensicauda , which would be related to a functional segregation of the olfactory system.