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TGF ‐β1 attenuated branching morphogenesis of embryonic murine submandibular gland through Smad3 activation
Author(s) -
Gao P.,
Qiao X.H.,
Gou L.M.,
Huang Y.,
Li Q.H.,
Li L.J.,
Wang X.Y.,
Li C.J.
Publication year - 2017
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12295
Subject(s) - morphogenesis , submandibular gland , embryonic stem cell , transforming growth factor , microbiology and biotechnology , salivary gland , embryo , small interfering rna , biology , embryogenesis , rna , immunology , endocrinology , gene , biochemistry
Summary Transforming growth factor‐β1 ( TGF ‐β1) plays several crucial regulatory roles in multiple physiological and pathological processes. The aim of this work was to investigate the role of TGF ‐β1 in branching morphogenesis of salivary gland. We harvested and cultured submandibular salivary glands ( SMG s) from murine embryos, which were then treated with exogenous TGF ‐β1, or its neutralized antibody, Smad3 inhibitor, or Smad3 small interfering RNA (si RNA ). Our results suggested that TGF ‐β1 attenuated branching morphogenesis of embryonic murine SMG via Smad3 activation, thus playing a negative regulatory role in salivary gland development.