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New Insights into the Neural Differentiation Potential of Canine Adipose Tissue‐Derived Mesenchymal Stem Cells
Author(s) -
Blecker D.,
Elashry M. I.,
Heimann M.,
Wenisch S.,
Arnhold S.
Publication year - 2017
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12270
Subject(s) - nestin , adipose tissue , glial cell line derived neurotrophic factor , biology , neurotrophic factors , stem cell , transplantation , neurosphere , neuroepithelial cell , mesenchymal stem cell , cellular differentiation , neural stem cell , adult stem cell , multipotent stem cell , microbiology and biotechnology , spinal cord injury , neuroscience , spinal cord , medicine , endocrinology , progenitor cell , biochemistry , receptor , gene
Summary Adipose tissue‐derived stem cells ( ASC s) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASC s have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASC s using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control. Morphological changes could already be noticed 1 h after neuronal induction. Gene expression analysis revealed that the neuronal markers nestin and β III ‐tubulin as well as MAP 2 were expressed after induction of neuronal differentiation. Additionally, the expression of the neurotrophic factors NGF , BDNF and GDNF was determined. Some of the neuronal markers and neurotrophic factors were already expressed in undifferentiated cells. Our findings point out that ASC s can reliably be differentiated into the neuronal lineage; therefore, these cells are a suitable cell source for cell transplantation in disorders of the central nervous system. Follow‐up studies would show the clinical benefit of these cells after transplantation.