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Rat Articular Cartilages Change Their Tissue and Protein Compositions During Perinatal Period
Author(s) -
KobayashiMiura M.,
Miura T.,
Osago H.,
Yamaguchi Y.,
Aoyama T.,
Tanabe T.,
Matsumoto K.i.,
Fujita Y.
Publication year - 2016
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12165
Subject(s) - extracellular matrix , glycosaminoglycan , femoral condyle , fetus , cartilage , articular cartilage , anatomy , matrix (chemical analysis) , chemistry , perinatal period , microbiology and biotechnology , andrology , biomedical engineering , materials science , biology , pathology , osteoarthritis , medicine , pregnancy , chromatography , genetics , alternative medicine
Summary Articular cartilage (AC) covers the surface of bones in joints and functions as a cushion against mechanical loading. The tissue contains abundant extracellular matrix (ECM), which mainly consists of proteoglycans (PG) and collagen (COL) fibres. The property of AC is gradually changing by ageing with gravity loading. To know the property change of AC by initial gravity loading during short period after birth, we performed histological assays and proteomics assay on the AC of the femoral condyle in knee joints of perinatal rats. The water content (%) was significantly decreased in neonate AC compared with fetal AC. During the perinatal stages (E19 and P0), the localizations of glycosaminoglycan (GAG) and type I and II COLs were homogeneous. The density of chondrocytes was significantly decreased in the deeper layers comparing with the surface layer in neonate AC. In addition, we found a drastic change in the protein expression pattern on proteomic analysis. The expressions of ECM components were relatively increased in neonate AC compared with fetal AC.