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Ultrastructure of Rabbit Embryos Exposed to Hyperthermia and Anti‐ H sp 70
Author(s) -
Olexikova L.,
Makarevich A. V.,
Pivko J.,
Chrenek P.
Publication year - 2013
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12014
Subject(s) - hyperthermia , perivitelline space , nucleolus , cytoplasm , embryo , ultrastructure , organelle , biology , lipid droplet , microbiology and biotechnology , mitochondrion , andrology , chemistry , anatomy , oocyte , medicine , paleontology , zona pellucida
Summary The aim of the study was to determine the effect of short‐term hyperthermia and H sp70 blockage on ultrastructural changes in cell organelles and nucleoli of rabbit preimplantation embryos. The embryos were cultured either at 37.5°C (control, C ) or 41.5°C (hyperthermia, HT ) during 6 h. The antibody against H sp70 was added into the culture medium (4 μg/ml) of morula stage embryos from C and HT groups. After termination of the culture, the embryos were processed for transmission electron microscopy. The embryos exposed to hyperthermia showed increased volume of lipid droplets, considerable occurrence of cellular debris in the perivitelline space and slight changes in the occurrence of microvilli on the surface of trophoblastic cells. In the embryos exposed to anti‐ H sp 70 at 37.5°C, there were considerable changes in mitochondria morphology, decreased volume of dense bodies in the cytoplasm and considerable changes in the occurrence of microvilli on the surface of trophoblastic cells. In the group of embryos exposed simultaneously to hyperthermia and anti‐ H sp 70, mitochondria were also expanded and swollen; the volume of flocculent vesicles and lipid droplets was increased and the volume of dense bodies in the cytoplasm was diminished. General organization of the cytoplasm in groups with anti‐ H sp70 was characterized by cell organelle segregation. Averaged size of the nucleolar area was significantly increased in the embryos exposed to hyperthermia, whereas in the group exposed to the anti‐ H sp70 without hyperthermia it was significantly diminished. Hyperthermia also caused disintegration of compact status of the nucleoli. In presence of anti‐ H sp 70, the structural changes, described within the nucleoli during hyperthermia, were not observed. In conclusion, these results document ultrastructural changes in cell organelles of rabbit preimplantation embryo caused by hyperthermia, and also changes in the nucleolar structures, at which presence of H sp‐70 inhibit these changes.