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Expression of DNA Methyltransferase Genes in Four‐Cell Bovine Embryos Cultured in the Presence of Oviductal Fluid
Author(s) -
Barrera D.,
García E. V.,
Sinowatz F.,
Palma G. A.,
JiménezDíaz M. A.,
Miceli D. C.
Publication year - 2013
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1111/ahe.12010
Subject(s) - dnmt1 , embryo , methyltransferase , dnmt3b , dna methyltransferase , biology , andrology , gene expression , gene , microbiology and biotechnology , chemistry , methylation , genetics , medicine
Summary The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase ( DNMT ) genes was examined in early bovine embryos (4‐cell stage). Real‐time quantitative PCR was performed to determine the relative expression of DNMT 1, DNMT 3a and DNMT 3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT 1 significantly increased when cultured with oviductal fluid, whereas DNMT 3a and DNMT 3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression.