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Comparison of morphology and genetic diversity between broodstock and hybrid offspring of oriental river prawn, Macrobrachium nipponense based on morphological analysis and SNP markers
Author(s) -
Li Yiming,
Fan Wujiang,
Huang Yinying,
Huang Yingying,
Du Xinglin,
Liu Zhiquan,
Huang Youhui,
Zhao Yunlong
Publication year - 2021
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.13081
Subject(s) - broodstock , biology , genetic diversity , population , zoology , genetic distance , genetics , genetic variation , aquaculture , fishery , gene , demography , sociology , fish <actinopterygii>
Summary Hybridization is an effective method for the genetic improvement of farmed species. In this study, three broodstock populations (Changjiang, CJ, Dongting, DT, and Dianshan, DS) of oriental river prawn, Macrobrachium nipponense were used, and DS was used as the female broodstock. Through three‐line hybridization, two hybrid populations were finally obtained. The F3 generation of the broodstock population and the F1 generation of the hybrid population were cultured indoors for 3 months. Through morphological analysis (cluster analysis, discriminant analysis and path analysis) it was found that the hybrid population and the broodstock had some differences, but not enough to reach the subspecies level, and the dominant traits exhibited differentiation and reorganization. This study identified SNP genetic markers, carried out systematic evolution analysis and genetic diversity analysis and found that the nucleotide diversity π and heterozygosity Het of the hybrid population were higher than those of the broodstock. Among broodstocks, the differentiation index ( F st ) of SCD and SDC was smallest (0.055). This research provides some valuable reference for genetic breeding.