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An association analysis for 14 candidate genes mapping to meat quality quantitative trait loci in a Duroc pig population reveals that the ATP 1A2 genotype is highly associated with muscle electric conductivity
Author(s) -
MármolSánchez E.,
Quintanilla R.,
Jordana J.,
Amills M.
Publication year - 2020
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12864
Subject(s) - single nucleotide polymorphism , biology , quantitative trait locus , genetics , population , genome wide association study , locus (genetics) , genotype , intramuscular fat , genetic association , candidate gene , purebred , gene , crossbreed , zoology , medicine , environmental health
Summary In previous GWAS carried out in a Duroc commercial line (Lipgen population), we detected on pig chromosomes 3, 4 and 14 several QTL for gluteus medius muscle redness ( GM a *), electric conductivity in the longissimus dorsi muscle ( LD CE ) and vaccenic acid content in the LD muscle ( LD C18:1 n − 7), respectively. We have genotyped, in the Lipgen population, 19 SNPs mapping to 14 genes located within these QTL . Subsequently, association analyses have been performed. After correction for multiple testing, two SNP s in the TGFBRAP 1 (rs321173745) and SELENOI (rs330820437) genes were associated with GM a *, whereas ACADSB (rs81449951) and GPR 26 (rs343087568) genotypes displayed significant associations with LD vaccenic content. Moreover, the polymorphisms located at the ATP 1A2 (rs344748241), ATP 8B2 (rs81382410) and CREB 3L4 (rs321278469 and rs330133789) genes showed significant associations with LD CE . We made a second round of association analyses including the SNP s mentioned above as well as other SNP s located in the chromosomes to which they map. After performing a correction for multiple testing, the only association that remained significant at the chromosome‐wide level was that between the ATP 1A2 genotype and LD CE . From a functional point of view, this association is meaningful because this locus encodes a subunit of the Na + /K + ‐ ATP ase responsible for maintaining an electrochemical gradient across the plasma membrane.