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The molecular structure and imprinting status of the IPW ( imprinted gene in the Prader‐Willi syndrome region ) gene in cattle
Author(s) -
Chen W.,
Xu D.,
Ma C.,
Zhang C.,
Li J.,
Zhang W.,
Zhao G.,
Li S.
Publication year - 2019
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12815
Subject(s) - biology , genomic imprinting , gene , exon , genetics , imprinting (psychology) , gene expression , microbiology and biotechnology , dna methylation
Summary IPW ( imprinted gene in the Prader‐Willi syndrome region ), a long non‐coding RNA , is a paternally expressed gene in the PWS / AS imprinted domain on human chromosome 15 and mouse chromosome 7. Disruption of the PWS / AS region is associated with three neurogenic disorders in humans. In this study, we identified the bovine homolog of the IPW gene; multiple transcripts obtained by RT ‐ PCR and RACE showed a complex and tissue‐specific expression pattern of IPW in the brain, heart, kidney, liver, lung, spleen and skeletal muscle. An informative single nucleotide polymorphism (rs133341090) in the long exon H was identified by sequencing the genomic DNA , and mono‐allelic expression of IPW was confirmed by sequencing the cDNA s of heterozygous individuals, indicating that IPW may be imprinted in cattle. The protein‐coding potential of IPW transcripts was assessed using coding potential calculator ( cpc ) software, which showed a negative score. In addition, sequencing analysis also indicated multiple small open reading frames in the bovine IPW transcript, but none of the ATG s was consistent with Kozak consensus. Taken together, the IPW transcripts are most likely long non‐coding RNA s.