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Genome‐wide identification and comparison of mRNA s, lnc RNA s and circ RNA s in porcine intramuscular, subcutaneous, retroperitoneal and mesenteric adipose tissues
Author(s) -
Liu X.,
Wei S.,
Deng S.,
Li D.,
Liu K.,
Shan B.,
Shao Y.,
Wei W.,
Chen J.,
Zhang L.
Publication year - 2019
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12781
Subject(s) - rna , adipose tissue , biology , messenger rna , gene expression , transcriptome , microbiology and biotechnology , rna silencing , rna editing , non coding rna , gene , genetics , rna interference , biochemistry
Summary Many types of RNA s, including messenger RNA s ( mRNA s), long noncoding RNA s (lnc RNA s) and circular RNA s (circ RNA s), play crucial roles in regulating fat cell differentiation and tissue development. However, the expression profiles of these RNA s in different adipose tissues are still largely unknown. To shed light on this issue, we performed a transcriptome analysis of mRNA s, lnc RNA s and circ RNA s obtained from intramuscular adipose tissue, subcutaneous adipose tissue, retroperitoneal adipose tissue and mesenteric adipose tissue of Chinese Erhualian pigs. A number of differentially expressed mRNA s, lnc RNA s and circ RNA s were identified among the four adipose tissues. Tissue‐specific analysis indicated that circ RNA s exhibited the highest tissue specificity among mRNA s, lnc RNA s and circ RNA s, whereas intramuscular adipose tissue had the most tissue‐specific genes among the four adipose tissues. Gene Ontology analysis showed that differentially expressed mRNA s among groups were involved mainly in lipid metabolism and immune inflammatory response processes. Furthermore, the co‐expression network construction of mRNA s–lnc RNA s revealed that several important lnc RNA s, such as MSTRG .426159 and MSTRG .604206, might associate with lipid metabolic process. Taken together, these data provide a genome‐wide resource of mRNA s, lnc RNA s and circ RNA s potentially involved in porcine fat metabolism, thus improving understanding of their function in diverse adipose tissues.