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A mini‐ STR typing system for degraded equine DNA
Author(s) -
Kun T. J.,
Wictum E. J.,
Penedo M. C. T.
Publication year - 2018
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12716
Subject(s) - biology , genotyping , amplicon , genetics , typing , allele , microsatellite , polymerase chain reaction , gene , microbiology and biotechnology , genotype
Summary Degraded biological samples are a challenge for testing laboratories. Genotyping success can be improved through the use of mini‐ STR s, by which primers are placed adjacent to the repeat motifs to reduce amplicon size. Here, we present a genetic profiling system comprising 13 autosomal and one X‐linked dinucleotide‐repeat markers and the SRY gene based on the internationally accepted equine parentage panel. The markers are divided into two panels with all alleles falling at or below 182 bp. The application of this method significantly increases the ability to profile difficult samples and to provide discriminating results to clients.