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PRLH and SOD 1 gene variations associated with heat tolerance in Chinese cattle
Author(s) -
Zeng L.,
Chen N.,
Ning Q.,
Yao Y.,
Chen H.,
Dang R.,
Zhang H.,
Lei C.
Publication year - 2018
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12702
Subject(s) - biology , heat index , genetics , allele , gene , isoleucine , genotype , missense mutation , leucine , amino acid , mutation , zoology , heat stress
Summary With the proposed global climate change, heat tolerance is becoming increasingly important to the sustainability of livestock production systems. Results from previous studies showed that variants in the prolactin releasing hormone ( PRLH ) ( AC _000160.1:g.11764610G>A) and superoxide dismutase 1 ( SOD 1 ) ( AC _000158.1:g.3116044T>A) genes play an important role in heat tolerance in African indicine cattle. However, it is unknown whether or not the mutations are associated with heat tolerance in Chinese cattle. In this study, PCR and DNA sequencing were used to genotype two missense mutations in 725 individuals of 30 cattle breeds. Analysis results demonstrated that two classes of base substitution were detected at two loci: AC _000160.1:g.11764610G>A and AC _000158.1:g.3116044T>A or T>C respectively, with amino acid substitutions arginine to histidine and phenylalanine to isoleucine or leucine. The frequencies of the G and T alleles of the two loci gradually diminished from northern groups to southern groups of native Chinese cattle, whereas the frequencies of A and A or C alleles showed a contrary pattern, displaying a significant geographical difference across native Chinese cattle breeds. Additionally, analysis of these two loci in Chinese indigenous cattle revealed that two SNP s were significantly associated with mean annual temperature (T), relative humidity ( RH ) and temperature humidity index ( THI ) ( P < 0.01), suggesting that cattle with A or C alleles were distributed in regions with higher T, RH and THI . Our results suggest that the two mutations of PRLH and SOD 1 genes in Chinese cattle were associated with the heat tolerance.