Premium
Discovery of porcine mi RNA ‐196a/b may influence porcine adipogenesis in longissimus dorsi muscle by mi RNA sequencing
Author(s) -
Liu Linqing,
Qian Kun,
Wang Chonglong
Publication year - 2017
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12520
Subject(s) - biology , adipogenesis , rna , microbiology and biotechnology , genetics , gene
Summary Intramuscular fat ( IMF ) is one of the fat traits that has economic importance in the pork industry. Longissimus dorsi muscle contains IMF and is suitable for studying adipogenesis. To discover further potential regulatory mi RNA s that may influence adipogenesis, we analyzed mi RNA in the longissimus dorsi muscle of Yorkshire ( YY , lean‐type) and Chinese Wannanhua ( WH , fatty) pigs using mi RNA sequencing (mi RNA ‐seq). From this dataset, we identified 598 unique mi RNA s comprising 325 pre‐mi RNA s and 273 novel pre‐mi RNA s through comparison with known mi RNA s in mi RB ase version 21. We found 42 mi RNA s including nine up‐ and 33 down‐regulated between the YY and WH pigs. Moreover, we found two mi RNA s, miR‐196a/b (miR‐196a, miR‐196b‐5p), that had the highest level of expression in WH pigs, and miR‐196a/b may influence porcine adipogenesis in longissimus dorsi muscle through an adipocytokine signaling pathway.