Premium
Identification of differentially expressed genes in longissimus muscle of pigs with high and low intramuscular fat content using RNA sequencing
Author(s) -
Lim K. S.,
Lee K. T.,
Park J. E.,
Chung W. H.,
Jang G. W.,
Choi B. H.,
Hong K. C.,
Kim T. H.
Publication year - 2017
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12518
Subject(s) - intramuscular fat , biology , transcriptome , rna seq , gene , tenderness , rna , genetics , gene expression , food science
Summary Intramuscular fat ( IMF ) content in pork is an important element of consumer preference and is positively correlated with meat quality, including tenderness and juiciness. With advances in RNA sequencing technologies, transcriptome‐related differences can be associated with specific traits in animals. The objective of this study was to investigate differentially expressed genes ( DEG s) closely related to IMF content in porcine longissimus muscle using RNA sequencing. A total of 107 Berkshire pigs were used for IMF content measurements, and significant differences between extremely high (H, n = 3) and low (L, n = 3) IMF content groups were found ( P < 0.0001). From multi‐dimensional scaling analyses, it was observed that the relationships between H and L groups were similar to each other. Here, we identified a total of 134 genes that were differentially expressed between the groups (false discovery rate <0.05; fold change ≥2). Functional analyses with DEG s revealed that lipid metabolism ( SCD and FASN ) was one of the significant biological processes related to IMF content determination. In addition, we found that DEG s related to muscle regeneration ( MYOG and VEGFA ) and extracellular matrix ( COL 1A1 , COL 1A2 , COL 5A1 , COL 14A1 and COL 15A1 ) were changed among individuals with extreme IMF contents. These results will aid in understanding the regulation of IMF content in pigs.