Premium
A genome‐wide association study of heat stress‐associated SNP s in catfish
Author(s) -
Jin Y.,
Zhou T.,
Geng X.,
Liu S.,
Chen A.,
Yao J.,
Jiang C.,
Tan S.,
Su B.,
Liu Z.
Publication year - 2017
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12482
Subject(s) - catfish , biology , genome wide association study , ictalurus , genetics , snp , single nucleotide polymorphism , gene , genetic association , genotype , fish <actinopterygii> , fishery
Summary Heat tolerance is a complex and economically important trait for catfish genetic breeding programs. With global climate change, it is becoming an increasingly important trait. To better understand the molecular basis of heat stress, a genome‐wide association study ( GWAS ) was carried out using the 250 K catfish SNP array with interspecific backcross progenies, which derived from crossing female channel catfish with male F1 hybrid catfish (female channel catfish × male blue catfish). Three significant associated SNP s were detected by performing an EMMAX approach for GWAS . The SNP located on linkage group 14 explained 12.1% of phenotypical variation. The other two SNP s, located on linkage group 16, explained 11.3 and 11.5% of phenotypical variation respectively. A total of 14 genes with heat stress related functions were detected within the significant associated regions. Among them, five genes— TRAF 2 , FBXW 5 , ANAPC 2 , UBR 1 and KLHL 29— have known functions in the protein degradation process through the ubiquitination pathway. Other genes related to heat stress include genes involved in protein biosynthesis ( PRPF 4 and SYNCRIP ), protein folding ( DNAJC25 ), molecule and iron transport ( SLC 25A46 and CLIC 5 ), cytoskeletal reorganization ( COL 12A1 ) and energy metabolism ( COX 7A2 , PLCB 1 and PLCB 4 ) processes. The results provide fundamental information about genes and pathways that is useful for further investigation into the molecular mechanisms of heat stress. The associated SNP s could be promising candidates for selecting heat‐tolerant catfish lines after validating their effects on larger and various catfish populations.