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Characterization of the NLRC 5 promoter in chicken: SNP s, regulatory elements and CpG islands
Author(s) -
Guo X. M.,
Liu X. P.,
Chang G. B.,
Xu L.,
Bi Y. L.,
Wang H. Z.,
Zhang Y.,
Zhu P. F.,
Wu Y.,
Chen G. H.
Publication year - 2016
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12450
Subject(s) - biology , cpg site , promoter , methylation , epigenetics , dna methylation , transcription factor , genetics , regulation of gene expression , gene , microbiology and biotechnology , gene expression
Summary NLRC 5 plays an important role in the innate immunity and cellular immunity in many species, but the regulatory mechanism of NLRC 5 expression in chickens remains unclear. In this study, a series of deletion fragments of the NLRC 5 promoter region were constructed and dual‐luciferase assay was performed. Then, we detected the SNP in the core region and its function. Important transcriptional regulatory elements were predicted and identified. Methylation of CpG islands was measured. The results revealed that the two core regions of −4372 to −3756 and −2925 to −2265 in the NLRC 5 promoter were essential for NLRC 5 mRNA expression in which a SNP (A/G), located at −2470, was found to have an effect on the transcriptional activity. Also, the STAT 1 element in the second core region of the NLRC 5 promoter was identified to bind with the STAT 1 transcription factor, which was necessary for the transcriptional activity. In addition, many other elements in the NLRC 5 promoter, including YY 1 and CEBP , may contribute significantly to the expression activity of NLRC 5 . Moreover, two CpG islands were searched. Part of one was located in the first core region, which suggests that epigenetic modification may regulate the activity of the first promoter region, and the other was mostly in an unmethylated state. Collectively, these results suggest the complex regulation of NLRC 5 expression includes SNP s, transcription factors and methylation.