Premium
Targeted high‐throughput growth hormone 1 gene sequencing reveals high within‐breed genetic diversity in South African goats
Author(s) -
Ncube K. T.,
Mdladla K.,
Dzomba E. F.,
Muchadeyi F. C.
Publication year - 2016
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12424
Subject(s) - biology , breed , genetic diversity , ecotype , genetics , population , boer goat , phylogenetic tree , growth hormone receptor , snp , gene , evolutionary biology , growth hormone , single nucleotide polymorphism , genotype , hormone , demography , endocrinology , sociology
Summary This study assessed the genetic diversity in the growth hormone 1 gene ( GH 1 ) within and between South African goat breeds. Polymerase chain reaction‐targeted gene amplification together with Illumina MiSeq next‐generation sequencing ( NGS ) was used to generate the full length (2.54 kb) of the growth hormone 1 gene and screen for SNP s in the South African Boer (SAB) ( n = 17), Tankwa ( n = 15) and South African village ( n = 35) goat populations. A range of 27–58 SNP s per population were observed. Mutations resulting in amino acid changes were observed at exons 2 and 5. Higher within‐breed diversity of 97.37% was observed within the population category consisting of SA village ecotypes and the Tankwa goats. Highest pairwise F ST values ranging from 0.148 to 0.356 were observed between the SAB and both the South African village and Tankwa feral goat populations. Phylogenetic analysis indicated nine genetic clusters, which reflected close relationships between the South African populations and the other international breeds with the exception of the Italian Sarda breeds. Results imply greater potential for within‐population selection programs, particularly with SA village goats.