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The micro RNA expression profile in porcine skeletal muscle is changed by constant heat stress
Author(s) -
Hao Y.,
Liu J. R.,
Zhang Y.,
Yang P. G.,
Feng Y. J.,
Cui Y. J.,
Yang C. H.,
Gu X. H.
Publication year - 2016
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12419
Subject(s) - biology , heat stress , constant (computer programming) , skeletal muscle , rna , stress (linguistics) , microbiology and biotechnology , food science , genetics , zoology , anatomy , gene , computer science , linguistics , philosophy , programming language
Summary Heat stress has profound effects on animal performance and muscle function, and micro RNA s (mi RNA s) play a critical role in muscle development and stress responses. To characterize the changes in mi RNA s in skeletal muscle responding to heat stress, the mi RNA expression profiles of longissimus dorsi muscles of pigs raised under constant heat stress (30 °C; n = 8) or control temperature (22 °C; n = 8) for 21 days were analyzed by Illumina deep sequencing. A total of 58 differentially expressed mi RNA s were identified with 30 down‐regulated and 28 up‐regulated, and 63 differentially expressed target genes were predicted by mi RNA – mRNA joint analysis. GO and KEGG analyses showed that the genes regulated by differentially expressed mi RNA s were enriched in glucose metabolism, cytoskeletal structure and function and stress response. Real‐time PCR showed that the mRNA levels of PDK 4, HSP 90 and DES were significantly increased, whereas those of SCD and LDHA significantly decreased by heat exposure. The protein levels of CALM 1, DES and HIF 1 α were also significantly increased by constant heat. These results demonstrated that the change in mi RNA expression in porcine longissimus dorsi muscle underlies the changes in muscle structure and metabolism in porcine skeletal muscle affected by constant heat stress.