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Expression profiling of the GBP 1 gene as a candidate gene for porcine reproductive and respiratory syndrome resistance
Author(s) -
Gol S.,
Estany J.,
Fraile L. J.,
Pena R. N.
Publication year - 2015
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12347
Subject(s) - biology , allele , genetics , locus (genetics) , gene , microbiology and biotechnology , genotype , candidate gene , untranslated region , porcine reproductive and respiratory syndrome virus , polyadenylation , three prime untranslated region , gene expression , messenger rna
Summary A genomic region in pig chromosome 4 has been previously associated with higher viraemia levels and lower weight gain following porcine reproduction and respiratory syndrome virus ( PRRSV ) infection. The region includes the marker WUR 1000125 , a G>A polymorphism next to a putative polyadenylation site in the 3’‐untranslated region (3’‐ UTR ) of the guanylate‐binding protein 1, interferon‐induced ( GBP 1 ) gene. The protein encoded by GBP 1 is a negative regulator of T‐cell responses. We show here that GBP 1 expression is lower in liver and tonsils of pigs carrying the WUR 1000125‐G allele due to differential allele expression (allele A expression is 1.9‐fold higher than for allele G ). We also show that the GBP 1 gene has two active polyadenylation signals 421 bp apart and that polyadenylation usage is dependent on the WUR 1000125 genotype. The distal site is the most prevalently used in all samples, but the presence of the A allele favours the generation of shorter transcripts from the proximal site. This is confirmed by a differential allele expression study in AG genotype liver and tonsil samples. The interaction between WUR 1000125 and other mutations identified in the 5’‐ and 3’‐ UTR regions of this gene needs to be studied. In conclusion, our study indicates that the WUR 1000125 mutation is associated with changes in the expression of the negative T‐cell regulator GBP 1 gene. However, the chromosome 4 locus for PRRSV viraemia levels and weight gain contains a cluster of four other GBP genes that remain to be studied as candidate genes for this QTL .