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Two mutations in the 5′‐flanking region of the KITLG gene are associated with litter size of dairy goats
Author(s) -
An X. P.,
Hou J. X.,
Lei Y. N.,
Gao T. Y.,
Song Y. X.,
Wang J. G.,
Cao B. Y.
Publication year - 2015
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12277
Subject(s) - biology , litter , single nucleotide polymorphism , genetics , genotype , primer (cosmetics) , genbank , gene , polymorphism (computer science) , zoology , chemistry , organic chemistry , agronomy
Summary In this study, Xinong Saanen ( SN ) and Guanzhong ( GZ ) dairy goat breeds were used to detect single nucleotide polymorphisms ( SNP s) in the 5′‐flanking region of the KITLG gene by DNA sequencing and primer‐introduced restriction analysis–polymerase chain reaction. Two novel SNP s (g.13090G>T and g.13664C>A) were identified (GenBank Accession no. KM 658964). Furthermore, g.13090G>T and g.13664C>A loci were closely linked in SN and GZ breeds ( r 2 > 0.33). Association analysis results showed that g.13090G>T and g.13664C>A SNP s significantly affected litter size ( P < 0.05). The litter size of individuals with the combined genotype GG / CC from both dairy goat breeds was greater than that of individuals with TT / AA in average parity ( P < 0.05). Known biochemical and physiological functions, along with our results, indicated that GG / CC could be used in marker‐assisted selection to choose individuals with greater litter size from both breeds. These results extend the spectrum of genetic variation in the caprine KITLG gene and may contribute to genetic resources and breeding of goats.