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Two missense mutations in exon 9 of caprine PRLR gene were associated with litter size
Author(s) -
Hou J. X.,
An X. P.,
Han P.,
Peng J. Y.,
Cao B. Y.
Publication year - 2015
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12223
Subject(s) - biology , prolactin receptor , single nucleotide polymorphism , haplotype , genetics , genotype , exon , litter , gene , missense mutation , polymorphism (computer science) , boer goat , microbiology and biotechnology , mutation , prolactin , endocrinology , hormone , agronomy
Summary Guanzhong ( n = 321) and Boer ( n = 191) goat breeds were used to detect single nucleotide polymorphisms ( SNP s) in the coding regions of the prolactin receptor ( PRLR ) gene by DNA sequencing and PCR‐RFLP . Two SNP s (c.1457G>A and c.1645G>A) were identified that caused amino acid variations p.Ser485Asn and p.Val548Met respectively. Statistical results indicated that the c.1457G>A and c.1645G>A SNPs were significantly associated with litter size in Boer and Guanzhong goat breeds. Further analysis revealed that combined genotype C4 ( GGGG ) and haplotype G‐G were better than the others for litter size in both goat breeds. These results might contribute to goat genetic resources and breeding.