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The differential expression of alternatively spliced transcripts and imprinting status of MEG9 gene in cows
Author(s) -
Zhang Kun,
Li Dongjie,
Wang Mengnan,
Wu Guojiang,
Shi Yunjiao,
Li Shijie
Publication year - 2014
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/age.12195
Subject(s) - biology , genomic imprinting , exon , gene , alternative splicing , genetics , rna splicing , gene isoform , imprinting (psychology) , complementary dna , gene expression , single nucleotide polymorphism , microbiology and biotechnology , genomic dna , rna , coding region , genotype , dna methylation
Summary Meg9 / Mirg ( maternally expressed gene 9/microRNA containing gene ), a non‐coding RNA (ncRNA) comprising many alternatively splicing isoforms, has been identified as maternally expressed in mouse and sheep, but its imprinting status and splicing variants are still unknown in cattle. In this study, we found three splicing variants of the cattle MEG9 gene expressed in a tissue‐specific manner. A single nucleotide polymorphism site (SNP c.1354C>G) was identified in exon 3 of cattle MEG9 and used to distinguish between monoallelic and biallelic expression. Our results showed that MEG9 exhibited monoallelic expression in all examined cattle tissues by comparing sequencing results between genomic DNA and cDNA levels at the c.1354C>G SNP site, suggesting that MEG9 is imprinted in cattle.